Molecular Sex Determination of Masked Lovebird (Agapornis personata) by Polymerase Chain Reaction Method

6 pages•Published: March 1, 2021

Shafira Inggrid El Islami, Medania Purwaningrum and Aris Haryanto

Abstract

Sex determination in monomorphic birds such as masked lovebird (Agapornis personata) based on physical appearance is difficult to conduct. It becomes a problem in the breeding program. Some rapid methods for sex determination have been developed, one of which is molecular sexing based on the amplification of Chromodomain Helicase DNA-binding (CHD) gene by polymerase chain reaction (PCR) method using specific primers. This method is a solution with rapid and accurate results. The aim of this study was to molecular sex determine of masked lovebird by PCR amplification. The feather samples were collected from ten masked lovebirds. DNA was isolated from the chalamus, part of the lovebird feather and it was used as a DNA template for PCR amplification. The PCR products then were electrophoresed in agarose gel and they were visualized by SYBRSafe staining on a UV transilluminator. It showed that a single DNA fragment in size of 400 bp generated for male masked lovebirds and double DNA fragments in size of 350 bp and 400 bp generated for female masked lovebirds.

Keyphrases: agapornis personata, chd gene, masked lovebird, pcr, rapid molecular sexing

In: Kustiati and Wolly Candramila (editors). Proceedings of KOBI 2nd International Conference on Management of Tropical Biodiversity for Human Welfare: From Ecosystem to Molecular, vol 1, pages 48-53.

Links:	https://easychair.org/publications/paper/N3Mt
	https://doi.org/10.29007/g21c

BibTeX entry

@inproceedings{KOBI-2019:Molecular_Sex_Determination_Masked,
  author    = {Shafira Inggrid El Islami and Medania Purwaningrum and Aris Haryanto},
  title     = {Molecular Sex Determination of Masked Lovebird (Agapornis personata) by Polymerase Chain Reaction Method},
  booktitle = {Proceedings of KOBI 2nd International Conference on Management of Tropical Biodiversity for Human Welfare: From Ecosystem to Molecular},
  editor    = {Kustiati and Wolly Candramila},
  series    = {EPiC Series in Biological Sciences},
  volume    = {1},
  publisher = {EasyChair},
  bibsource = {EasyChair, https://easychair.org},
  issn      = {2398-5291},
  url       = {/publications/paper/N3Mt},
  doi       = {10.29007/g21c},
  pages     = {48-53},
  year      = {2021}}

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