Advancing Infectious Disease Research: Development and Characterization of Human Skin and Immune System (hSIS) Humanized Mouse and Rat Models

EasyChair Preprint no. 12436

Abstract

The human skin, as the primary barrier against pathogen transmission, is a key focus in infectious disease research. Despite the utility of rodent models in studying human-specific skin pathogens, achieving successful co-engraftment of human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of a novel human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, involving the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells. The human skin, as the primary barrier against pathogen transmission, is a key focus in infectious disease research. Despite the utility of rodent models in studying human-specific skin pathogens, achieving successful co-engraftment of human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of a novel human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, involving the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells.

Keyphrases: Human skin, Humanized Mouse, infectious disease

@Booklet{EasyChair:12436,
  author = {Liis Tammik},
  title = {Advancing Infectious Disease Research: Development and Characterization of Human Skin and Immune System (hSIS) Humanized Mouse and Rat Models},
  howpublished = {EasyChair Preprint no. 12436},

  year = {EasyChair, 2024}}