Advancements in Preclinical Research: Humanized Mouse and Rat Models Integrating Full-Thickness Human Skin and Autologous Immune Cells

EasyChair Preprint 12461

Abstract

The use of animal models in biomedical research has been instrumental in advancing our understanding of human physiology, disease pathology, and therapeutic interventions. However, the inherent differences between human and animal immune systems pose challenges in accurately predicting human-specific responses to diseases and treatments. The development of humanized mouse and rat models, incorporating full-thickness human skin and autologous immune cells, represents a significant stride towards bridging this gap. This paper explores the methodology, applications, and potential impact of these advanced models on preclinical research and drug development. The human skin, as the primary barrier against pathogen transmission, is a key focus in infectious disease research. Despite the utility of rodent models in studying human-specific skin pathogens, achieving successful co-engraftment of human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of a novel human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, involving the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells.

Keyphrases: Humanized rodent models, Immunodeficient mice, Skin infections

@booklet{EasyChair:12461,
  author    = {Liis Tammik},
  title     = {Advancements in Preclinical Research: Humanized Mouse and Rat Models Integrating Full-Thickness Human Skin and Autologous Immune Cells},
  howpublished = {EasyChair Preprint 12461},
  year      = {EasyChair, 2024}}